6G0B
Crystal Structure of a GH8 xylotriose complex from Teredinibacter Turnerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.721, 80.522, 87.986 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.470 - 1.800 |
R-factor | 0.16831 |
Rwork | 0.167 |
R-free | 0.19743 |
Starting model (for MR) | 6g00 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.511 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.470 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 40092 | |
<I/σ(I)> | 8.5 | |
Completeness [%] | 100.0 | |
Redundancy | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.1 M sodium acetate, pH 5, 0.2 M ammonium sulfate, 20% PEG 6000 |