6FV8

Dimer structure of the MATE family multidrug resistance transporter Aq_128 from Aquifex aeolicus in the outward-facing state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-07
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	80.100, 73.600, 101.800
Unit cell angles	90.00, 99.80, 90.00

Refinement procedure

Resolution	19.926 - 3.000
R-factor	0.2659
Rwork	0.265
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fv6
RMSD bond length	0.003
RMSD bond angle	0.631
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.130	0.712
Rmeas	0.150	0.840
Rpim	0.074	0.442
Number of reflections	23304	2291
<I/σ(I)>	9.1
Completeness [%]	99.0	97.9
Redundancy	3.6	3.5
CC(1/2)	0.993	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	8	295	0.1 M Tris 0.2 M ammonium sulphate 30% PEG 500 MME(v/v)