6FK9
Crystal structure of N2C/D282C stabilized opsin bound to RS09
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 243.193, 243.193, 111.688 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.630 - 2.630 |
R-factor | 0.2362 |
Rwork | 0.235 |
R-free | 0.26070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j4q |
RMSD bond length | 0.005 |
RMSD bond angle | 0.713 |
Data reduction software | XDS ((VERSION Oct 15) |
Data scaling software | Aimless |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.630 | 2.730 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmerge | 0.137 | 1.008 |
Number of reflections | 37496 | |
<I/σ(I)> | 12.48 | 0.53 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.51 | 11.93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | see publication |