6FCQ
The X-ray Structure of Lytic Transglycosylase Slt inactive mutant E503Q from Pseudomonas aeruginosa in complex with Bulgecin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97953 |
Spacegroup name | P 63 |
Unit cell lengths | 165.715, 165.715, 55.884 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 143.510 - 3.100 |
R-factor | 0.18313 |
Rwork | 0.181 |
R-free | 0.21683 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ohu |
RMSD bond length | 0.007 |
RMSD bond angle | 1.261 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 143.510 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rpim | 0.037 | |
Number of reflections | 15755 | |
<I/σ(I)> | 16.53 | |
Completeness [%] | 97.1 | |
Redundancy | 16.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 80mM Tris pH 8.5, 12% PEG 8000 and 160mM calcium acetate |