6FBI
KlenTaq DNA polymerase in a closed, ternary complex with dGpNHpp bound in the active site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-12 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 109.770, 109.770, 91.460 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.532 - 1.900 |
R-factor | 0.2086 |
Rwork | 0.207 |
R-free | 0.23520 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.713 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12rc1_2815: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.532 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmeas | 0.120 | 2.445 |
Number of reflections | 97132 | 7175 |
<I/σ(I)> | 14.61 | 1.3 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 14.95 | 14.58 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | (A) 16 % PEG 4000, 0.1 M sodium acetate, 20 mM manganese(II) chloride, 0.1 M MES (B) 17 % PEG 4000, 0.1 M ammonium acetate, 0.1 M HEPES, 20 mM manganese(II) chloride (C) 15 % PEG 4000, 0.1 M sodium acetate, 20 mM manganese(II) chloride, 0.1 M MES |