6FBF
KlenTaq DNA polymerase processing a modified primer - bearing the modification upstream at the fourth primer nucleotide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 109.310, 109.310, 91.123 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.333 - 2.001 |
| R-factor | 0.2011 |
| Rwork | 0.200 |
| R-free | 0.22560 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.523 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12rc1_2815: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.333 | 2.011 |
| High resolution limit [Å] | 2.001 | 2.001 |
| Rmeas | 0.058 | 0.661 |
| Number of reflections | 81905 | 13044 |
| <I/σ(I)> | 19.53 | 2.87 |
| Completeness [%] | 99.9 | 98.1 |
| Redundancy | 5.36 | 5.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | 16 % PEG 4000, 20 mM manganese(II) chloride, 0.1 M TRIS, 0.1 M sodium acetate |
