6F6O
Structure of Adenovirus 3 fiber head V239D mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-15 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.873 |
Spacegroup name | P 21 3 |
Unit cell lengths | 81.867, 81.867, 81.867 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.890 - 1.490 |
R-factor | 0.15228 |
Rwork | 0.151 |
R-free | 0.16984 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wyj |
RMSD bond length | 0.018 |
RMSD bond angle | 1.818 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.800 | 1.580 |
High resolution limit [Å] | 1.490 | 1.490 |
Rmeas | 0.040 | 0.329 |
Number of reflections | 27755 | 3062 |
<I/σ(I)> | 23.6 | 2.8 |
Completeness [%] | 92.2 | 63.9 |
Redundancy | 4.6 | |
CC(1/2) | 0.990 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% PEG3350, HEPES, pH 7.4 |