6F2I
Crystal structure of Hen Egg-White Lysozyme co-crystallized in presence of 100 mM Tb-Xo4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97973 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.149, 78.149, 37.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.070 - 1.200 |
R-factor | 0.174 |
Rwork | 0.173 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h87 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.080 |
High resolution limit [Å] | 1.200 |
Rpim | 0.029 |
Number of reflections | 37176 |
<I/σ(I)> | 12.4 |
Completeness [%] | 99.7 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Sodium acetate pH 4.6, 0.2 to 0.8 M Chlorure de Sodium. Tb-Xo4 was directly mixed with the protein solution at a final concentration of 100 mM prior to crystallization. |