6EHP
The crystal structure of the human LAMTOR complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-20 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.8726 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 133.896, 133.896, 75.984 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.300 |
R-factor | 0.19942 |
Rwork | 0.198 |
R-free | 0.22758 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.187 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.072 | 1.440 |
Rpim | 0.027 | 0.543 |
Number of reflections | 256155 | |
<I/σ(I)> | 16.6 | |
Completeness [%] | 99.7 | 97.1 |
Redundancy | 8.2 | |
CC(1/2) | 0.990 | 0.570 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% [w/v] PEG 3350, 0.1M Tris/HCl pH8.5, 0.2M MgCl2, 3% 2-Methyl-2-propanol |