6E8I
Legionella Longbeachae LeSH (Llo2327) bound to phosphotyrosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 114 |
Detector technology | IMAGE PLATE |
Collection date | 2013-05-28 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.810, 64.140, 67.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.990 - 1.680 |
R-factor | 0.2185 |
Rwork | 0.217 |
R-free | 0.25030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6e8h |
RMSD bond length | 0.006 |
RMSD bond angle | 0.776 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.970 | 1.760 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.058 | 0.166 |
Number of reflections | 18762 | 2479 |
<I/σ(I)> | 8.5 | 4 |
Completeness [%] | 85.4 | 80.6 |
Redundancy | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 278 | PEG3000, sodium acetate, Tris-HCl |