6DKS
Structure of the Rbpj-SHARP-DNA Repressor Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2012-02-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.978 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.450, 231.570, 90.260 |
Unit cell angles | 90.00, 99.88, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.780 | 2.800 |
Rmerge | 0.084 | 0.480 |
Number of reflections | 54503 | 3903 |
<I/σ(I)> | 14.9 | |
Completeness [%] | 99.3 | 95.14 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.6 | 277 | Bis-Tris pH6.6 100mM NaCl 40% PEG400 200mM NDSB-256 |