6D86
Structure of the Bovine p85a BH domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2015-10-25 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.643, 91.260, 93.324 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.800 - 2.301 |
R-factor | 0.1963 |
Rwork | 0.195 |
R-free | 0.21430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.783 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.800 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.069 | 0.426 |
Number of reflections | 33073 | 1627 |
<I/σ(I)> | 31.4 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.4 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.5 M Li2SO4, 100 mM Na Cacodylate, pH 6.0 |