6D2Z
Structure of human Usb1 with uridine-adenosine, inactive H208Q mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.393, 51.045, 46.299 |
Unit cell angles | 90.00, 105.88, 90.00 |
Refinement procedure
Resolution | 44.530 - 1.180 |
R-factor | 0.147 |
Rwork | 0.145 |
R-free | 0.17380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5v1m |
RMSD bond length | 0.023 |
RMSD bond angle | 1.735 |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.530 | 1.200 |
High resolution limit [Å] | 1.180 | 1.180 |
Number of reflections | 55920 | |
<I/σ(I)> | 14 | |
Completeness [%] | 91.6 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 289 | 100 mM bis-tris, 25% SOKALAN CP 42, 1.5% tetrahydrofuran |