6CZM
Crystal structure of Medicago truncatula ATP-phosphoribosyltransferase in tense form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.170, 178.660, 92.110 |
Unit cell angles | 90.00, 105.71, 90.00 |
Refinement procedure
Resolution | 43.295 - 2.880 |
R-factor | 0.192 |
Rwork | 0.191 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Domains of MedtrATP-PRT1 in relaxed form |
RMSD bond length | 0.007 |
RMSD bond angle | 1.033 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.880 | 2.880 |
Rmerge | 0.047 | 0.800 |
Rmeas | 0.055 | 0.930 |
Number of reflections | 62118 | 4580 |
<I/σ(I)> | 18.4 | 1.8 |
Completeness [%] | 99.1 | 99.5 |
Redundancy | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 292 | histidine and AMP (5 mM each) were added to the protein solution supplemented with 10 mM MgCl2 and 100 mM KCl and incubated for 1 h. Reservoir solution: 2.53 M NaCl, 100 mM Bis-Tris propane pH 6.3 and 10% glycerol |