6CZL
Crystal structure of Medicago truncatula ATP-phosphoribosyltransferase in relaxed form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-09 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.9780 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 99.826, 195.621, 100.209 |
Unit cell angles | 90.00, 95.85, 90.00 |
Refinement procedure
Resolution | 39.170 - 2.920 |
R-factor | 0.198 |
Rwork | 0.197 |
R-free | 0.25000 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.286 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.090 |
High resolution limit [Å] | 2.920 | 2.920 |
Rmerge | 0.067 | 0.900 |
Rmeas | 0.076 | 1.050 |
Number of reflections | 82460 | 12719 |
<I/σ(I)> | 15.2 | 1.8 |
Completeness [%] | 99.1 | 95.3 |
Redundancy | 4.2 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 292 | 2.63 M NaCl, 100 mM Bis-Tris propane pH 6.3 |