6CEZ

Crystal Structure of Rabbit Anti-HIV-1 gp120 V2 Fab 16C2 in complex with V2 peptide ConB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.979460
Spacegroup name	P 1 21 1
Unit cell lengths	64.145, 41.470, 79.893
Unit cell angles	90.00, 91.68, 90.00

Refinement procedure

Resolution	39.929 - 2.399
R-factor	0.2402
Rwork	0.237
R-free	0.29580
RMSD bond length	0.002
RMSD bond angle	0.425
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.930	2.540
High resolution limit [Å]	2.400	2.400
Rmeas	0.061	0.690
Number of reflections	32149	2679
<I/σ(I)>	20.2	2.16
Completeness [%]	99.8	99.4
Redundancy	3.71	3.73
CC(1/2)	0.999	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	23% polyethylene glycol 3350, 0.2 M LiCL, 0.1 M 2-ethanesulfonic acid (MES) pH 6.5