6C9F

AMP-activated protein kinase bound to pharmacological activator R734

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	123.289, 123.289, 406.594
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.671 - 2.924
R-factor	0.2292
Rwork	0.228
R-free	0.24490
RMSD bond length	0.003
RMSD bond angle	0.719
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.060
High resolution limit [Å]	2.920	2.920
Number of reflections	40620
<I/σ(I)>	14.5
Completeness [%]	99.9
Redundancy	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M tri-sodium acetate pH 5.6, 0.2 M ammonium acetate, 15% w/v PEG 4000