6C8K

RNA-activated 2-AIpG monomer complex, 30 min soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	99
Detector technology	CCD
Collection date	2017-10-25
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	P 3 2 1
Unit cell lengths	48.103, 48.103, 82.197
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.259
R-factor	0.22735
Rwork	0.223
R-free	0.30223
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dhc
RMSD bond length	0.020
RMSD bond angle	2.624
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.091	0.354
Rmeas	0.101	0.396
Rpim	0.042	0.175
Number of reflections	4919	508
<I/σ(I)>	22	2.22
Completeness [%]	92.3	55.2
Redundancy	5.3	4.3
CC(1/2)	0.988	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	10% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.040 M Sodium cacodylate trihydrate pH 7.0, 0.012 M Spermine tetrahydrochloride, 0.080 M Sodium chloride, 0.020 M Magnesium chloride