6BTG
Crystal structure of deoxyribose-phosphate aldolase bound with DHAP from Bacillus Thuringiensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-04-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9786 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 105.104, 105.104, 49.366 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.981 - 1.698 |
R-factor | 0.1883 |
Rwork | 0.186 |
R-free | 0.22460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c24 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.029 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.981 | 1.759 |
High resolution limit [Å] | 1.698 | 1.698 |
Rmerge | 0.060 | 0.571 |
Rmeas | 0.061 | 0.061 |
Number of reflections | 31070 | 3017 |
<I/σ(I)> | 41.77 | 6.74 |
Completeness [%] | 99.8 | 98.43 |
Redundancy | 28.2 | 27.9 |
CC(1/2) | 1.000 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 200 mM NaCl, 26 % (v/v) PEG-3350, 100 mM HEPES pH 7.5 |