6BPA
Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to monoclonal antibody 3E9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 59.070, 177.100, 178.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.051 - 2.530 |
R-factor | 0.1947 |
Rwork | 0.194 |
R-free | 0.22460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w53 5en2 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.632 |
Data reduction software | iMOSFLM (7.2.1) |
Data scaling software | Aimless (0.2.7) |
Phasing software | PHENIX (1.12_2829) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.540 | 2.590 |
High resolution limit [Å] | 2.530 | 2.530 |
Number of reflections | 63529 | 4411 |
<I/σ(I)> | 9.9 | 1.9 |
Completeness [%] | 99.8 | 99.3 |
Redundancy | 6.7 | 6.4 |
CC(1/2) | 0.993 | 0.552 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 0.2 M KBr, 15% (w/v) PEG 4,000 and 0.05 M sodium citrate/citric acid |