6BLG
Crystal Structure of Sugar Transaminase from Klebsiella pneumoniae Complexed with PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 130.853, 130.853, 138.752 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.767 - 2.102 |
| R-factor | 0.1774 |
| Rwork | 0.175 |
| R-free | 0.22530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zah |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.749 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.770 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 26700 | 1324 |
| <I/σ(I)> | 18.3 | 1.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.6 | 6.2 |
| CC(1/2) | 0.748 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 1.6M Ammonium sulfate, 0.1M MES monohydrate pH 6.5, 10% v/v 1,4-Dioxane |
