6BKT
Crystal structure of the A/Michigan/15/2014 (H3N2) influenza virus hemagglutinin in complex with 6'-SLN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | H 3 2 |
Unit cell lengths | 100.354, 100.353, 394.281 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.17508 |
Rwork | 0.174 |
R-free | 0.19992 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o5n |
RMSD bond length | 0.012 |
RMSD bond angle | 1.525 |
Data scaling software | HKL-2000 (712) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rpim | 0.020 | 0.360 |
Number of reflections | 71063 | 7073 |
<I/σ(I)> | 33 | 1.6 |
Completeness [%] | 99.1 | 100 |
Redundancy | 7.3 | 7.6 |
CC(1/2) | 1.000 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1 M Tris pH 8.5, 0.2 M lithium sulfate, and 40% PEG 300 |