6BJX

Group I self-splicing intron P4-P6 domain mutant U131A (with isopropanol soaking)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979100
Spacegroup name	P 21 21 21
Unit cell lengths	74.670, 130.010, 146.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.800 - 3.140
R-factor	0.213003674255
Rwork	0.211
R-free	0.22924
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gid
RMSD bond length	0.003
RMSD bond angle	0.633
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall
Low resolution limit [Å]	63.800
High resolution limit [Å]	3.140
Number of reflections	25244
<I/σ(I)>	16.3
Completeness [%]	98.9
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 1000, sodium cacodylate, spermine