6B6Z
Crystal structure of the Apo Antibody fragment (Fab) raised against C-terminal domain of Ebola nucleoprotein (EBOV, TAFV, BDBV strains)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-25 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.000 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 74.621, 74.621, 323.695 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.974 - 2.112 |
R-factor | 0.2053 |
Rwork | 0.203 |
R-free | 0.25760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vkd |
RMSD bond length | 0.004 |
RMSD bond angle | 0.800 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.400 | 2.150 |
High resolution limit [Å] | 2.110 | 2.110 |
Number of reflections | 50593 | 2442 |
<I/σ(I)> | 4.23 | |
Completeness [%] | 93.7 | |
Redundancy | 6.2 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 288 | 180 mM Zinc Acetate, 90 mM imidazole, pH 8.0, 27% PEG 3000, 10 mM Chromium (III) chloride hexahydrate |