6B5R
Structure of PfCSP peptide 21 with human antibody CIS42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.131, 70.570, 165.379 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.949 - 1.775 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.23710 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.780 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 26.949 |
High resolution limit [Å] | 1.770 |
Number of reflections | 35657 |
<I/σ(I)> | 15 |
Completeness [%] | 74.1 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% PEG3350, 9% Isopropanol, 0.12M Ammonium Citrate pH 8.5 |