6B4I
Crystal structure of human Gle1 CTD-Nup42 GBM-DDX19B(ADP) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0333 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.740, 74.650, 146.830 |
| Unit cell angles | 90.00, 94.85, 90.00 |
Refinement procedure
| Resolution | 46.091 - 3.619 |
| R-factor | 0.2387 |
| Rwork | 0.234 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.069 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2006: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.900 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rpim | 0.061 | 0.833 |
| Number of reflections | 21685 | 2157 |
| <I/σ(I)> | 8.5 | 1 |
| Completeness [%] | 98.6 | 98.9 |
| Redundancy | 6.9 | 7 |
| CC(1/2) | 0.997 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 13 % (w/v) PEG 3350, 0.2 M sodium potassium phosphate |
