6AVK
Streptavidin bound to peptide-like compound KPM-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9774 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 47.310, 93.520, 104.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.670 - 1.400 |
R-factor | 0.174 |
Rwork | 0.174 |
R-free | 0.18600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vwa |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.650 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.042 | 0.337 |
Rmeas | 0.050 | 0.416 |
Rpim | 0.027 | 0.239 |
Number of reflections | 45941 | 2212 |
<I/σ(I)> | 18 | 3.4 |
Completeness [%] | 100.0 | 98.3 |
Redundancy | 5.8 | 4.5 |
CC(1/2) | 0.999 | 0.861 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.2 M potassium iodide, 20% PEG3350 |