6A87
Pholiota squarrosa lectin (PhoSL) in complex with fucose(alpha1-6)GlcNAc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2017-05-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 145.778, 145.778, 39.531 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.460 - 2.410 |
R-factor | 0.18028 |
Rwork | 0.177 |
R-free | 0.23703 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6a86 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.588 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.086 | 0.486 |
Number of reflections | 11922 | |
<I/σ(I)> | 36.7 | 4.4 |
Completeness [%] | 98.1 | |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.8 M potassium-sodium phosphate, 5% 1,3-butanediol |