5ZWJ
Crystal structure of EGFR 675-1022 T790M/C797S/V948R in complex with EAI045
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-20 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.91905 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 79.586, 79.586, 215.075 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.686 - 2.900 |
R-factor | 0.2559 |
Rwork | 0.255 |
R-free | 0.27180 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.392 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 10084 | |
<I/σ(I)> | 20.1 | |
Completeness [%] | 99.9 | |
Redundancy | 17.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 26% PEG 4000, 0.1 M KCl, 0.05 M Tris pH 7.5-8.0 |