5ZS3
Small heat shock protein from M. marinum:Form-1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | I 2 3 |
Unit cell lengths | 91.140, 91.140, 91.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.446 - 2.001 |
R-factor | 0.1984 |
Rwork | 0.196 |
R-free | 0.24530 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.226 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.450 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.121 | |
Rpim | 0.052 | 0.531 |
Number of reflections | 8114 | 634 |
<I/σ(I)> | 14.1 | 3.4 |
Completeness [%] | 93.4 | 100 |
Redundancy | 12.3 | 11 |
CC(1/2) | 0.997 | 0.825 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 293 | 2.1 M DL-Malic acid pH 7.0 |