5ZOI
Crystal Structure of alpha1,3-Fucosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-27 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.988 |
| Spacegroup name | P 32 |
| Unit cell lengths | 121.664, 121.664, 64.718 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.800 - 3.190 |
| R-factor | 0.288 |
| Rwork | 0.287 |
| R-free | 0.31600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.397 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.800 | 3.340 |
| High resolution limit [Å] | 3.180 | 3.180 |
| Number of reflections | 17862 | 3213 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.8 | |
| Redundancy | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | peg4000 |
