5ZNT
Insect chitin deacetylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97776 |
Spacegroup name | I 4 |
Unit cell lengths | 136.006, 136.006, 77.209 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.085 - 1.979 |
R-factor | 0.1683 |
Rwork | 0.167 |
R-free | 0.19070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zns |
RMSD bond length | 0.010 |
RMSD bond angle | 0.994 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.010 |
High resolution limit [Å] | 1.979 | 1.979 |
Number of reflections | 49155 | 2407 |
<I/σ(I)> | 4.3 | |
Completeness [%] | 100.0 | |
Redundancy | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 281 | 0.2M Sodium malonate pH 7.0, 20% w/v Polyethylene glycol 3350 |