5ZMP
The structure of a lysine deacylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 |
Unit cell lengths | 56.790, 56.790, 112.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.190 |
Rwork | 0.173 |
R-free | 0.21490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g11 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.700 | 2.250 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.214 | 1.130 |
Rpim | 0.132 | 0.681 |
Number of reflections | 18344 | 1342 |
<I/σ(I)> | 8.7 | 2.2 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 7 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.26 M Sodium phosphate monobasic monohydrate, 0.14 M Potassium phosphate dibasic |