5ZM4
Fe(II)/(alpha)ketoglutarate-dependent dioxygenase AndA with preandiloid C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2017-12-11 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 |
Unit cell lengths | 62.594, 67.218, 73.317 |
Unit cell angles | 88.06, 89.78, 89.94 |
Refinement procedure
Resolution | 45.817 - 1.950 |
R-factor | 0.1862 |
Rwork | 0.184 |
R-free | 0.22370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ybm |
RMSD bond length | 0.008 |
RMSD bond angle | 0.911 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.700 | 1.990 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.080 | 0.569 |
Number of reflections | 84526 | 4428 |
<I/σ(I)> | 9.3 | |
Completeness [%] | 97.1 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% (w/v) PEG 3350, 200mM sodium tartrate, 20% DMF |