5ZHY
Structural characterization of the HCoV-229E fusion core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.108, 77.348, 77.386 |
| Unit cell angles | 76.89, 89.78, 90.20 |
Refinement procedure
| Resolution | 31.006 - 2.441 |
| R-factor | 0.2277 |
| Rwork | 0.224 |
| R-free | 0.26320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ieq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.047 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.039 | 0.235 |
| Number of reflections | 24629 | 1186 |
| <I/σ(I)> | 17.2 | 2.9 |
| Completeness [%] | 97.9 | 94.4 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.5 M Ammonium sulfate, 12% (v/v) Glycerol, 100 mM Tris/HCl, PH 8.5 |
