5Z74
Crystal structure of alkaline/neutral invertase InvB from Anabaena sp. PCC 7120 complexed with sucrose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97916 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 166.946, 82.171, 97.446 |
Unit cell angles | 90.00, 119.13, 90.00 |
Refinement procedure
Resolution | 31.350 - 1.950 |
R-factor | 0.20978 |
Rwork | 0.208 |
R-free | 0.23854 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5z73 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.114 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.096 | 0.537 |
Number of reflections | 80206 | 8173 |
<I/σ(I)> | 13.1 | 2.4 |
Completeness [%] | 95.7 | 98.1 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 0.7M lithium chloride, 6% polyethylene glycol 6000, 0.1M MES, pH 6.0 |