5YYX
Crystal Structure of the MEK1 FHA domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97736 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.666, 61.666, 61.078 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.204 - 1.684 |
| R-factor | 0.1847 |
| Rwork | 0.183 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g6g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.906 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.720 |
| High resolution limit [Å] | 1.680 | 4.140 | 1.680 |
| Rmerge | 0.082 | 0.051 | 0.589 |
| Rmeas | 0.084 | 0.053 | 0.606 |
| Rpim | 0.019 | 0.012 | 0.140 |
| Total number of observations | 303239 | ||
| Number of reflections | 15632 | 1134 | 936 |
| <I/σ(I)> | 4.7 | ||
| Completeness [%] | 99.4 | 100 | 91.1 |
| Redundancy | 19.4 | 17.9 | 18.3 |
| CC(1/2) | 0.999 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2% PEG8000, 0.1M Imidazole malate pH7.5, 1.0M Lithium sulfate |
