5YUC

Crystal structure of voltage-gated sodium channel NavAb N49K mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-05
Detector	RAYONIX MX225-HS
Wavelength(s)	1
Spacegroup name	I 4 2 2
Unit cell lengths	127.440, 127.440, 202.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.175 - 3.401
R-factor	0.234673895642
Rwork	0.230
R-free	0.27868
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yua
RMSD bond length	0.011
RMSD bond angle	1.173
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.180	3.523
High resolution limit [Å]	3.400	3.400
Rmerge	0.250
Rpim	0.071
Number of reflections	11809
<I/σ(I)>	13.6
Completeness [%]	87.3
Redundancy	13.6
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	293	11% polyethylene glycol monomethyl ether 2000, 100 mM NaCl, 100 mM calcium nitrate, 100 mM Tris-HCl, pH 8.4