5YUA
Crystal structure of voltage-gated sodium channel NavAb in high-pH condition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-31 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 126.450, 126.450, 201.658 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.461 - 2.801 |
R-factor | 0.231669922293 |
Rwork | 0.230 |
R-free | 0.26213 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rvy |
RMSD bond length | 0.010 |
RMSD bond angle | 1.325 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.470 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.248 | 0.314 |
Rpim | 0.087 | 1.074 |
Number of reflections | 20186 | 505 |
<I/σ(I)> | 14.02 | |
Completeness [%] | 87.7 | 25.29 |
Redundancy | 9.1 | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | 11% polyethylene glycol monomethyl ether 2000, 100 mM NaCl, 100 mM calcium nitrate, 100 mM Tris-HCl pH 8.4 |