5YK9
Crystal structure of selenomethionine-labelled indole prenyltransferase AmbP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2017-02-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97921 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.259, 142.350, 157.147 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.160 - 3.001 |
R-factor | 0.2401 |
Rwork | 0.239 |
R-free | 0.26470 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.170 |
Data scaling software | Aimless |
Phasing software | SHELXDE |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.160 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.122 | 0.513 |
Rmeas | 0.556 | |
Rpim | 0.213 | |
Number of reflections | 21912 | 2545 |
<I/σ(I)> | 17.6 | |
Completeness [%] | 99.4 | 98.7 |
Redundancy | 7.1 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG8000, MES, magnesium chloride |