5YIX
Caulobacter crescentus GcrA sigma-interacting domain (SID) in complex with domain 2 of sigma 70
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 295.15 |
Detector technology | PIXEL |
Collection date | 2016-05-29 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 65.199, 65.199, 397.934 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.061 - 2.302 |
R-factor | 0.224 |
Rwork | 0.222 |
R-free | 0.25650 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.947 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.110 | 0.750 |
Rmeas | 0.120 | 0.780 |
Rpim | 0.030 | 0.200 |
Number of reflections | 23503 | 2028 |
<I/σ(I)> | 29.1 | 2.8 |
Completeness [%] | 98.9 | 82 |
Redundancy | 20 | 14.6 |
CC(1/2) | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 18% PEG 3350, 0.1M sodium citrate, pH 5.5 |