5YH1
Member of s1p family of ribosomal proteins PF0399 DHH domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 132.500, 132.500, 212.245 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.250 - 2.180 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.20200 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.130 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.180 |
Number of reflections | 56239 |
<I/σ(I)> | 15 |
Completeness [%] | 97.0 |
Redundancy | 33.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 4% (V/V) PEG 400 100 mM Sodium acetate/ Acetic acid pH 5.5 2000 mM Lithium sulfate 100 mM Magnesium sulfate |