5YGU
Crystal structure of Escherichia coli (strain K12) mRNA Decapping Complex RppH-DapF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.418, 86.418, 173.093 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.985 - 2.298 |
R-factor | 0.2077 |
Rwork | 0.206 |
R-free | 0.23870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4s2v 4ijz |
RMSD bond length | 0.009 |
RMSD bond angle | 1.030 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.290 |
Number of reflections | 30026 |
<I/σ(I)> | 18.8 |
Completeness [%] | 99.8 |
Redundancy | 14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | Potassium Sodium Tartrate, Sodium Iodine, Xylitol |