5YB0
Crystal Structure of Wild Type Phosphoserine aminotransferase (PSAT) from E. histolytica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77.15 |
Detector technology | CCD |
Collection date | 2011-04-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 |
Unit cell lengths | 66.446, 134.073, 139.064 |
Unit cell angles | 62.85, 88.58, 74.92 |
Refinement procedure
Resolution | 50.150 - 2.940 |
R-factor | 0.267 |
Rwork | 0.265 |
R-free | 0.30100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bt4 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.553 |
Data reduction software | HKL-2000 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.150 | 3.050 |
High resolution limit [Å] | 2.940 | 3.000 |
Rmerge | 0.083 | 0.881 |
Number of reflections | 76771 | |
<I/σ(I)> | 14.911 | |
Completeness [%] | 94.2 | 85.8 |
Redundancy | 3.7 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289.15 | 25-30% PEG 3350 (w/v), 100 mM Tris pH 6.5, 200mM sodium formate, 5 % glycerol (v/v) |