5Y8Q

ZsYellow at pH 8.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-26
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9795
Spacegroup name	P 21 2 21
Unit cell lengths	48.410, 72.883, 123.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 2.900
R-factor	0.1774
Rwork	0.174
R-free	0.23613
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ogr
RMSD bond length	0.013
RMSD bond angle	1.767
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.900
Number of reflections	9367
<I/σ(I)>	18.33
Completeness [%]	92.0
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.5	Tris