5Y61
YfiB-YfiR complexed with GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.595, 58.422, 69.869 |
| Unit cell angles | 107.39, 97.28, 89.90 |
Refinement procedure
| Resolution | 42.207 - 2.990 |
| R-factor | 0.1997 |
| Rwork | 0.197 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eb1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.242 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.210 | 3.050 |
| High resolution limit [Å] | 2.990 | 3.000 |
| Number of reflections | 14631 | 746 |
| <I/σ(I)> | 11.3 | 2.33 |
| Completeness [%] | 97.8 | 98.3 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1 M Tris-HCl (pH 8.5) , 8% w/v polyethylene glycol 8000 |
