5XZX
Crystal structure of importin-alpha3 bound to the nuclear localization signal of Ran-binding protein 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2011-02-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.970, 60.220, 85.020 |
Unit cell angles | 90.00, 100.54, 90.00 |
Refinement procedure
Resolution | 37.130 - 3.000 |
R-factor | 0.2292 |
Rwork | 0.227 |
R-free | 0.28070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uae |
RMSD bond length | 0.002 |
RMSD bond angle | 0.502 |
Data scaling software | Aimless (0.5.32) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.860 | 48.860 | 3.180 |
High resolution limit [Å] | 3.000 | 9.000 | 3.000 |
Rmerge | 0.053 | 0.022 | 0.268 |
Rmeas | 0.064 | 0.026 | 0.318 |
Rpim | 0.034 | 0.014 | 0.168 |
Total number of observations | 33080 | ||
Number of reflections | 9597 | ||
<I/σ(I)> | 12 | ||
Completeness [%] | 99.1 | 94.5 | 99.6 |
Redundancy | 3.4 | 3.3 | 3.5 |
CC(1/2) | 0.998 | 0.999 | 0.977 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 8000 |