5XWP
Crystal structure of LbuCas13a-crRNA-target RNA ternary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-21 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9785 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 95.576, 132.870, 139.960 |
Unit cell angles | 90.00, 90.80, 90.00 |
Refinement procedure
Resolution | 48.179 - 3.086 |
R-factor | 0.2197 |
Rwork | 0.218 |
R-free | 0.24370 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.594 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.130 |
High resolution limit [Å] | 3.080 | 3.080 |
Rmerge | 0.185 | 0.558 |
Number of reflections | 61255 | |
<I/σ(I)> | 14 | 3.5 |
Completeness [%] | 98.6 | 98.6 |
Redundancy | 15 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 M HEPES, pH 7.5, 0.1 M L-Proline, 8% PEG 3350 |