5XSF
Crystal structure of the 2-keto-3-deoxy-6-phosphogluconate aldolase of Zymomonas mobilis ZM4 with 3-phosphoglycerate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-14 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.00 |
Spacegroup name | F 41 3 2 |
Unit cell lengths | 186.299, 186.299, 186.299 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.853 - 1.962 |
R-factor | 0.1916 |
Rwork | 0.191 |
R-free | 0.21210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xse |
RMSD bond length | 0.004 |
RMSD bond angle | 0.658 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.853 | 1.990 |
High resolution limit [Å] | 1.962 | 1.960 |
Number of reflections | 20372 | |
<I/σ(I)> | 17.8 | 3.4 |
Completeness [%] | 99.5 | 100 |
Redundancy | 13.2 | 18.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.6M Ammonium Sulfate, 0.1M CAPS (pH 10.5), 0.2M Lithium Sulfate |