5XPK

Crystal structure of ubiquitin-k6mimic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-28
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 1 2 1
Unit cell lengths	26.656, 46.626, 40.495
Unit cell angles	90.00, 91.47, 90.00

Refinement procedure

Resolution	40.482 - 2.274
R-factor	0.2543
Rwork	0.250
R-free	0.31030
RMSD bond length	0.003
RMSD bond angle	0.663
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.482	2.340
High resolution limit [Å]	2.274	2.300
Number of reflections	4160
<I/σ(I)>	9.39	1.16
Completeness [%]	97.3	80
Redundancy	2.9	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Ammonium formate, 20% w/v PEG 3350